By M. Ahearne, Alicia J. El Haj, S. Rauz (auth.), Alicia El Haj, Dan Bader (eds.)
This quantity provides chosen peer-reviewed papers of the eighth foreign convention on phone & Stem mobilephone Engineering (ICCM) 2010 in Dublin. The contributions are written by way of prime scientists in mobile and Stem phone Engineering and the themes of the papers contain:
Computational phone Mechanics
Experimental strategies in mobile Mechanics
Molecular and mobilephone Imaging
Cell Matrix Interactions
Mechanotransduction and phone mechanics
Stem mobilephone area of interest
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Medium was fully replaced twice a week, with 500µl samples taken from wells for each group (n=2-3) at each medium exchange. B. Dynamic Compression Application Intermittent dynamic compression (DC) was carried out in a custom pneumatic based compression bioreactor housed within an incubator as previously described (14). 01 N/construct preload at a frequency of 1Hz. Constructs were loaded each day for 2 consecutive hours, 5days/week over 42 days. Free swelling (FS) controls were maintained adjacent to the bioreactor during loading periods, in the same amount of medium.
As therapeutically high numbers of MSCs need to be grown from a limited number of cells that can be harvested from tissue, there is an unmet need to increase the expansion efficiency of MSCs in a critical time window. Aging poses a smaller problem in hESCs, however, they tend to differentiate fast and uncontrollably under suboptimal culture conditions and lose their potential rapidly. To maintain their pluripotency ESCs must be grown on feeder cells (murine or human fibroblasts) [3, 4]. This is technically difficult and impedes downstream analysis and applications.
Dynamically compressed microchanneled groups (DCM) accumulated comparable levels of sGAG as IFMBE Proceedings Vol. 30 28 T. Mesallati et al. 64 µg vs. 2 µg). We also noted that there was no significant difference in sGAG levels between the four groups at the final time point when normalized to DNA (Fig. 1D). 44 µg) after 42 days in culture (Fig. 1C). We next compared core and annulus accumulation at day 42 (Fig. 3A). All groups were found to have significantly greater sGAG levels in the annulus compared to their corresponding core (µg).